Anti-DMC1 antibody [2H12/4] ab11054 from Abcam

Product Specs
ItemAnti-DMC1 antibody [2H12/4]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab11054
Antibody TypePrimary
IsotypeIgG2a
Quantity100 UG
ApplicationsFlow Cytometry
Clone2H12/4
ClonalityMonoclonal
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1.2 mg/ml
Research AreaEpigenetics
Gene NameDMC1
ReactivityHuman
TargetDMC1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Three-color dSTORM Imaging and Analysis of Recombination Foci in Mouse Spread Meiotic NucleiBIO-PROTOCOLJuly 20, 2023Lieke Koornneef, Maarten W. Paul, Adriaan B. Houtsmuller, Willy M. Baarends, Johan A. Slotman(Sigma-Aldrich, catalog number: 70166), storage temperature: RTMouse monoclonal anti-DMC1 (Abcam, catalog number: ab11054), aliquot storage temperature: -80 °C, stock concentration: 1 mg/mL Note: After thawing an aliquot,

(2) Multi-color dSTORM microscopy in Hormad1-/- spermatocytes reveals alterations in meiotic recombination intermediates and synaptonemal complex structurePLOS GeneticsJuly 20, 2022Lieke Koornneef, Johan A. Slotman, Esther Sleddens-Linkels, Wiggert A. van Cappellen, Marco Barchi, Attila Tóth, Joost Gribnau, Adriaan B. Houtsmuller, Willy M. Baarendsanti-RAD51 (1:1000, [81]), anti-SYCP1 (1:2500, [82]) and mouse monoclonal anti-DMC1 (1:1000, Abcam, ab11054), anti-SYCP3(1:200, Abcam, ab97672), For secondary antibodies, we used goat anti-guinea pig IgG Alexa

(3) Production of functional sperm from in vitro-cultured premeiotic spermatogonia in a marine fishZoological ResearchJuly 18, 2022Hong Zhang et al.3D+Hor+2-Iod, and 3D+Hor+Mel+Luz. ERK1/2 (ET160129, Huabio, China), p-ERK1/2 (ET160322, Huabio, China), DMC1 (ab11054, Abcam, UK), and β-actin (T56715S, Abmart, China) were examined by western blotting as described previously

(4) BRCA2 binding through a cryptic repeated motif to HSF2BP oligomers does not impact meiotic recombinationNature communicationsJuly 29, 2021Ghouil R, Miron S, Koornneef L, Veerman J, Paul MW, Le Du MH, Sleddens-Linkels E, van Rossum-Fikkert SE, van Loon Y, Felipe-Medina N, et al.Gold with DAPI (Invitrogen). The following primary antibodies were used: mouse anti-DMC1 (1:1000,Abcam ab11054), mouse anti-SYCP3 (1:200, Abcam ab97672), mouse anti-MLH1 (1:25, BD Pharmingen 51-1327GR), and rabbit

(5) The SUN1-SPDYA interaction plays an essential role in meiosis prophase INature communicationsMay 26, 2021Chen Y, Wang Y, Chen J, Zuo W, Fan Y, Huang S, Liu Y, Chen G, Li Q, Li J, et al.A2228, 1:2000, RRID: AB_476697), GAPDH (Proteintech, 60004-1, 1:2000, RRID: ab_2107436), DMC1 (Abcam, ab11054, 1:500, RRID: AB_297706), Lamin B1 (Proteintech, 66095-1, 1:1000, RRID: AB_11232208), CDK2 (Santa cruz,

(6) Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesisTheranosticsJanuary 1, 2021Wang M, Xu Y, Zhang Y, Chen Y, Chang G, An G, Yang X, Zheng C, Zhao J, Liu Z, et al.(Abcam, ab32363), rabbit anti-Kit to mouse (Cell Signaling Technology, 3074), mouse anti-DMC1 (Abcam, ab11054), rabbit anti-SYCP3 (Abcam, ab15093), chicken anti-GFP (Abcam, ab13970), rabbit anti-UTF1 (Abcam, ab105090),

(7) The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination IntermediatesMolecular cellAugust 20, 2020Hinch AG, Becker PW, Li T, Moralli D, Zhang G, Bycroft C, Green C, Keeney S, Shi Q, Davies B, et al.,Thermo Fisher Scientific,"Cat# A-21235, RRID:AB_2535804" Mouse monoclonal anti-DMC1 clone 2H12/4,Abcam,"Cat# ab11054, RRID:AB_297706" Mouse polyclonal anti-RAD51,Abcam,"Cat# ab88572, RRID:AB_2042762" Rabbit polyclonal

(8) Super-resolution imaging of RAD51 and DMC1 in DNA repair foci reveals dynamic distribution patterns in meiotic prophasePLoS geneticsJune 1, 2020Slotman JA, Paul MW, Carofiglio F, de Gruiter HM, Vergroesen T, Koornneef L, van Cappellen WA, Houtsmuller AB, Baarends WM.antibodies, we used goat antibody anti-SYCP3 (R&D Systems), mouse monoclonal antibody anti-DMC1 (Abcam ab11054), and a previously generated rabbit polyclonal anti-RAD51 [40]. For secondary antibodies, we used a

(9) Mitotic Slippage and Extranuclear DNA in Cancer Chemoresistance: A Focus on TelomeresInternational journal of molecular sciencesApril 16, 2020Salmina K, Bojko A, Inashkina I, Staniak K, Dudkowska M, Podlesniy P, Rumnieks F, Vainshelbaum NM, Pjanova D, Sikora E, et al.USA" DMC1,Mouse monoclonal,"Specific for DMC1, does not cross-react with the related protein Rad51",1:100,"ab11054, Abcam, Cambridge, UK" F-ACTIN,,Phalloidin-iFlour 594 Conjugate,1:500,"ab176757, Abcam, Cambridge,

(10) Exogenous Gonadotrophin Stimulation Induces Partial Maturation of Human Sertoli Cells in a Testicular Xenotransplantation Model for Fertility PreservationJournal of Clinical MedicineJanuary 18, 2020Marsida Hutka, Lee B. Smith, Ellen Goossens, W. Hamish B. Wallace, Jan-Bernd Stukenborg, Rod T. Mitchell(Spermatogonia),Gift #,-,1:200 γH2AX,Germ cell ** (meiotic),Abcam,AB26350,1:1000 DMC1,Germ cell (meiotic),Abcam,AB11054,1:4000 BOLL,Germ cell (meiotic),Novus Bio,H00066037-M03,1:1000 Ki67,Proliferating cell,Abcam,AB16667,1:100 c-PARP,Apoptotic

(11) SIRT7 promotes chromosome synapsis during prophase I of female meiosisChromosomaSeptember 1, 2019Berta N. Vazquez, Cecilia S. Blengini, Yurdiana Hernandez, Lourdes Serrano, Karen Schindler(1:1000, Abcam, ab155176), rat anti-RPA (1:150, Cell Signaling, 2208T), mouse anti-DMC1 (1:2000, Abcam ab11054), mouse anti-MLH1 (1:50, BD Pharmingen, 550838) and rabbit anti-H3K18ac (1:1000, Abcam, ab11911). The

(12) The meiotic TERB1-TERB2-MAJIN complex tethers telomeres to the nuclear envelopeNature communicationsFebruary 4, 2019Wang Y, Chen Y, Chen J, Wang L, Nie L, Long J, Chang H, Wu J, Huang C, Lei M.(Santa Cruz, sc-40, 1:1000), FLAG (Sigma, F3165, 1:200), actin (Sigma, A2228, 1:2000), DMC1 (Abcam, ab11054, 1:100), Lamin B1 (Proteintech, 66095-1-Ig, 1:500), CDK2 (Santa Cruz, sc-6248, 1:100). Secondary antibody

(13) The Cancer Aneuploidy Paradox: In the Light of EvolutionGenes (Basel)January 25, 2019Kristine Salmina, Anda Huna, Martins Kalejs, Dace Pjanova, Harry Scherthan, Mark S. Cragg, Jekaterina ErenpreisaUSA DMC1 Mouse monoclonal Specific for DMC1—does not cross-react with the related protein Rad51 1:100 ab11054, Abcam Cambridge, UK LAMIN B1 Goat polyclonal Peptide mapping at the C-terminus of Lamin B1 of human

(14) Urban fine particulate matter exposure causes male reproductive injury through destroying blood-testis barrier (BTB) integrityToxicology lettersJanuary 15, 2017Cao XN, Shen LJ, Wu SD, Yan C, Zhou Y, Xiong G, Wang YC, Liu Y, Liu B, Tang XL, et al.

(15) Damaging legacy: maternal cigarette smoking has long-term consequences for male offspring fertilityHuman reproduction (Oxford, England)December 1, 2014Sobinoff AP, Sutherland JM, Beckett EL, Stanger SJ, Johnson R, Jarnicki AG, McCluskey A, St John JC, Hansbro PM, McLaughlin EA.

(16) Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomesPLoS geneticsJuly 1, 2014Jessica Hopkins, Grace Hwang, Justin Jacob, Nicklas Sapp, Rick Bedigian, Kazuhiro Oka, Paul Overbeek, Steve Murray, Philip W Jordan

(17) SPO11-Independent DNA Repair Foci and Their Role in Meiotic SilencingPLoS GeneticsJune 6, 2013Fabrizia Carofiglio et al.

(18) Ikbkap/Elp1 Deficiency Causes Male Infertility by Disrupting Meiotic ProgressionPLoS GeneticsMay 23, 2013Fu-Jung Lin, Li Shen, Chuan-Wei Jang, Pål Ø. Falnes, Yi Zhang

(19) Meiotic functions of RAD18Journal of Cell ScienceAugust 15, 2011Akiko Inagaki et al.

(20) The role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cellsExperimental cell researchSeptember 10, 2009Erenpreisa J, Cragg MS, Salmina K, Hausmann M, Scherthan H.

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Anti-DMC1 antibody [2H12/4] from Abcam

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Anti-DMC1 antibody [2H12/4] from Abcam

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